Spectrophotometric determination of ascorbic acid concentration

Bromate Solutions of bromates acidified with nitric acid 1 in 20yield a white, crystalline precipitate with the addition of 2 or 3 drops of silver nitrate TS, which dissolves by heating. A pale yellow precipitate is produced with the addition of 1 drop of sodium nitrite TS.

Spectrophotometric determination of ascorbic acid concentration

Theoretical background[ edit ] The acid dissociation constant for an acid is a direct consequence of the underlying thermodynamics of the dissociation reaction; the pKa value is directly proportional to the standard Gibbs free energy change for the reaction. The value of pKa also depends on molecular structure of the acid in many ways.

For example, Pauling proposed two rules: Other structural factors that influence the magnitude of the acid dissociation constant include inductive effectsmesomeric effectsand hydrogen bonding.


Hammett type equations have frequently been applied to the estimation of pKa. In particular, the pH of a solution can be predicted when the analytical concentration and pKa values of all acids and bases are known; conversely, it is possible to calculate the equilibrium concentration of the acids and bases in solution when the pH is known.

These calculations find application in many different areas of chemistry, biology, medicine, and geology.

For example, many compounds used for medication are weak acids or bases, and a knowledge of the pKa values, together with the water—octanol partition coefficientcan be used for estimating the extent to which the compound enters the blood stream. Acid dissociation constants are also essential in aquatic chemistry and chemical oceanographywhere the acidity of water plays a fundamental role.

In living organisms, acid—base homeostasis and enzyme kinetics are dependent on the pKa values of the many acids and bases present in the cell and in the body. In chemistry, a knowledge of pKa values is necessary for the preparation of buffer solutions and is also a prerequisite for a quantitative understanding of the interaction between acids or bases and metal ions to form complexes.

Experimentally, pKa values can be determined by potentiometric pH titrationbut for values of pKa less than about 2 or more than about 11, spectrophotometric or NMR measurements may be required due to practical difficulties with pH measurements.

The equilibrium constant for this dissociation reaction is known as a dissociation constant.

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Acetic acida weak aciddonates a proton hydrogen ion, highlighted in green to water in an equilibrium reaction to give the acetate ion and the hydronium ion.

The acid loses a proton, leaving a conjugate base; the proton is transferred to the base, creating a conjugate acid.

Acids and bases are thus regarded simply as donors and acceptors of protons respectively. A broader definition of acid dissociation includes hydrolysisin which protons are produced by the splitting of water molecules.

According to Lewis 's original definition, an acid is a substance that accepts an electron pair to form a coordinate covalent bond.Spectrophotometric analysis of total ascorbic acid contetnt in various For the determination of ascorbic acid in food concentration of ascorbic acid in .

An acid dissociation constant, K a, (also known as acidity constant, or acid-ionization constant) is a quantitative measure of the strength of an acid in feelthefish.com is the equilibrium constant for a chemical reaction known as dissociation in the context of acid–base reactions.

In aqueous solution, the equilibrium of acid dissociation can be written symbolically as. Spectrophotometric analysis of total ascorbic acid contetnt in various spectrophotometric determination of total ascorbic acid based on coupling with acidic DNPH in twenty-one concentration of ascorbic acid in the investigated samples.

Spectrophotometric determination of ascorbic acid concentration

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The relative contributions of ascorbic acid and phenolic antioxidants to the total antioxidant activity of orange and apple fruit juices and blackcurrant drink. Mar 29,  · Ascorbic Acid Determination in Commercial Fruit Juice Samples by Cyclic Voltammetry.

A method was developed for assessing ascorbic acid concentration in commercial fruit juice by cyclic voltammetry. Tütem E, Özyürek M, Apak R. Spectrophotometric determination of ascorbic acid using copper(II)-neocuproine .

Spectrophotometric determination of ascorbic acid concentration
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